ABSTRACT
Aim: Since the impact of H. pylori and its virulence is not clear in GERD, this study aimed to evaluate the prevalence of cag A and cag E gens of H. pylori among Iranian GERD patients
Background: Gastroesophageal reflux disease [GERD] is defined as a condition of reflux the stomach juice by low pH causes tissue damage. Helicobacter pylori may or may not influence the GERD; however, it is unclear
Methods: This study was a case-control study performed on patients with GERD who underwent upper gastrointestinal endoscopy at Taleghani Hospital of Tehran, Iran. Prevalence of H. pylori and presence of the cag A and cag E genes in GERD and control group was investigated
Results: H. pylori was detected in 54% and 62% of GERD and control groups respectively. Prevalence of cag A gene among GERD patients was 44.4% whereas among the control group it was 87%. Prevalence of the cag E among GERD patients and control group was 44.4% and 64% respectively. Coexistence of cag A and cag E in GERD patients was 25.7% and in the control patients it was 54.8%
Conclusion: We did not find correlation between H. pylori existence in GERD patients in comparison to the control group. Similar to other Asian studies, the presence of the cag A in control group was more than GERD patients significantly. The co-existence of cag A and cag E was also more in control group significantly
ABSTRACT
Enterotoxigenic Escherichia coli [ETEC] is the most important bacterial cause of watery travelers' diarrhea in developing countries. Watery diarrhea is can cause serious life-threatening dehydration. ETEC was caused diarrhea by the secretion of two heat-labile enterotoxins [LTs] and the heat-stable enterotoxins [STs] which increase intestinal secretion. Routine laboratory methods are not appropriate to detect ETEC and other diarrheagenic E. coli pathotypes. The molecular techniques such as PCR are rapid and accurate methods that have been developed for detection of ETEC. We were recognized ETEC by PCR on lt and st genes from E. coli isolates from patients with diarrhea collected from selected Tehran educational hospitals. The E. coli isolates were collected from total 140 patients with diarrhea and 110 patients without diarrhea using culture and IMViC test. DNA was extracted by boiling method and the presence of the uidA, lt and st genes was detected by PCR. Among 140 E. coli isolates from diarrheal stools 5 [3.6%] isolates were positive for, just lt gene, 3 [2.1%] co-amplified for both lt/st and 1 [0.7%] was positive for just the st gene which were considered as ETEC. In the E. coli isolates from non-diarrheal control samples just one [0.9%] isolate was positive for both lt and st genes. The results showed that the ETEC as a significant cause of diarrhea, usually ignored by laboratories using traditional methods. Sometimes the ETEC causes severe diarrhea and can threaten for patient's life. Thus a rapid diagnostic test such as PCR can be very helpful in the treatment of patients
ABSTRACT
We aimed for detection of bacterial DNA [bactDNA] in spontaneous bacterial peritonitis [SBP] by polymerase chain reaction [PCR] and its prognostic relevance in cirrhotic patients with culture-negative non-neutrocytic ascites [CNNNA]. approximately 60% of patients with spontaneous bacterial peritonitis [SBP] are ascites culture negative. Of each 77 patients with cirrhosis and ascites, two samples including blood and ascitic fluid [AF] were taken. Blood samples were obtained for routine biochemical study and PMN count. AF samples were used for biochemical analysis and aerobic and anaerobic culture. BactDNA was detected by polymerase chain reaction [PCR] using bacterial universal 16srRNA gene primer. Of all AF samples, 3 [3.9%] were positive for bacterial culture [one streptococcus a hemolytic and two E.coli]. The mean number of PMN in AF was 63. BactDNA was detected in 33 [42.9%] of 77 of samples [group A] and bactDNA was absent in 41 [53.2%] of samples [group B]. Blood WBC, prothrombin time, LDH, serum total protein, AF WBC, serum albumin, AF albumin, AF total protein, serum total bilirubin, AST, ALT and BUN were not statically different among group A and B. Hepatitis B, 41[45%], was the most frequent cause of cirrhosis. Hepatitis B is the common cause of cirrhosis in Iranian cirrhotic patients. Also, current study showed that high number of Iranian cirrhotic patients with CNNNA carries bactDNA in their AF. The clinical findings as well as clinical laboratory data in patients with CNNNA are independent to bactDNA status in their ascitic fluid
ABSTRACT
An association between Chlamydia pneumoniae [C. pneumoniae] and cardiovascular disease has been demonstrated. In this study, we aimed to study this potential relationship in 105 Iranian patients. Coronary artery specimens from 105 Iranian patients undergoing CABG were analyzed by PCR method for C. pneumoniae. Serological evaluation for C. pneumoniae IgG and IgM was performed using ELISA. 53 specimens from mamillary artery were also investigated. C. pneumoniae PCR test result was positive for 23 [21.9%] of patients with coronary artery atherosclerosis, but none of the specimens from the mamillary artery was positive for C. pneumoniae when it was evaluated by the PCR [P<0.001]. Coronary artery disease patients with and without a history of unstable angina or myocardial infarction were comparable in C. pneumoniae PCR test positive rates [P=0.618]. Relevance of IgG and IgM positivity were also studied by correlating it to the study parameters, but no difference was found. CRP was significantly higher in the IgM positive group [P<0.001]. A significant proportion of coronary atherosclerotic plaques are infected with C. pneumoniae while no infection was found in the normal mamillary artery specimens. No association was found between acute coronary syndromes and serological and PCR positivity. Further prospective randomized controlled studies with large patient population are needed to confirm our findings
ABSTRACT
Helicobacter pylori is an important pathogen for gastroduodenal diseases. Infection with H. pylori can be limited by regimens of multiple antimicrobial agents. However, antibiotic resistance is a leading cause of treatment failure. The aim of this study has been to determine the resistance patterns of H. pylori strains isolated from gastric biopsies of patients with dyspepsia by agar dilution method, in Tehran, Iran. H. pylori isolates from patients with gastrointestinal diseases were evaluated for susceptibility testing by agar dilution method. Susceptibility testing was performed to commonly used antibiotics including clarithromycin, tetracycline, amoxicillin, metronidazole and ciprofloxacin. Among 92 patients with dyspepsia, H. pylori strains were isolated from 42 patients. Seventeen [40.5%] of the isolates were resistant to metronidazole [MICs >/= 8 microg/1], whereas one isolate [2.4%] was resistant to amoxicillin [MICs
Subject(s)
Humans , Male , Female , Microbial Sensitivity Tests , Anti-Bacterial Agents , Dyspepsia/microbiology , Drug Resistance, Microbial , Clarithromycin , Tetracycline , Amoxicillin , Metronidazole , Ciprofloxacin , Polymerase Chain ReactionABSTRACT
Celiac disease has been reported to be associated with gastric abnormalities. The aim of this study was to assess the relationship between the prevalence of celiac disease and Helicobacter pylori infection in an Iranian population of 250 patients. Biopsies were taken from the gastric antrum and duodenum. Morphology and histology were evaluated using the updated Sydney system and modified Marsh criteria, respectively. To simplify the interpretation of gastric lesions we classified gastritis in macroscopic and microscopic stages. Serology for anti-tissue transglutaminase antibody was performed to determine the presence of celiac disease. Among 250 patients, 232 [93%] had histological evidence of Helicobacter pylori infection. Histological abnormalities [Marsh I to IIIc] were present in 24 [10%]. Of 24 patients, 20 [83%] with histological abnormalities were infected with Helicobacter pylori. Of 250 patients, 25 [10%] had a positive anti-tissue transglutaminase antibody. Of 25 anti-tissue transglutaminase antibody positive patients, 9 [3.6%] had microscopic and macroscopic enteritis [Marsh I to IIIc]. Clinical presentation of celiac disease was not distinguishable from cases infected with Helicobacter pylori. Histology, even in patients with positive serology, was non-specific and unhelpful. We found a high prevalence of Helicobacter pylori infection and chronic gastritis, but neither was associated with celiac disease, in agreement with studies in Western populations